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Thursday, July 16, 2020 | History

3 edition of Insertion and replication of Pseudomonas aeruginosa mutator phage D3112 found in the catalog.

Insertion and replication of Pseudomonas aeruginosa mutator phage D3112

Shehnaz Rehmat

Insertion and replication of Pseudomonas aeruginosa mutator phage D3112

by Shehnaz Rehmat

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  • 3 Currently reading

Published in 1984 .
Written in English


Edition Notes

StatementShehnaz Rehmat.
Classifications
LC ClassificationsMicrofilm 84/140 (Q)
The Physical Object
FormatMicroform
Paginationviii, 158 leaves : ill.
Number of Pages158
ID Numbers
Open LibraryOL2961717M
LC Control Number84205951

Figure 1 In vitro steps before phage use for in vitro or in vivo models. Notes: (A) Specific bacterial activity: the Pseudomonas aeruginosa strains of interest (eg, Strain 1 and Strain 2) are co-cultured on agar plates with a phage suspension to verify the lytic activity of the phages against known bacterial strains. (B) Phage suspension is titrated with the double agar overlay plaque . Characterization of bacteriophages infecting clinical isolates of Pseudomonas aeruginosa stored in a culture collection C.C.S. Zanetti1, R.C.C. Mingrone1, J.J. Kisielius2, M. Ueda-Ito2 and A.C.C. Pignatari1 1Laborato ´rio Especial de Microbiologia Clınica, Divisa ˜o de Doenc¸as Infecciosas, Universidade Federal de Sao Paulo, Sa˜o Paulo, SP, Brasil.

Results show that phage selection produces an evolutionary trade-off in MDR P. aeruginosa, whereby the evolution of bacterial resistance to phage attack changes the efflux pump mechanism, causing increased sensitivity to drugs from several antibiotic classes. The emergence of antibiotic-resistant bacterial strains has become a global crisis and is vulnerable for the exploration of alternative antibacterial therapies. The present study emphasizes the use of bacteriophage for the treatment of multidrug resistant P. aeruginosa. P. aeruginosa was used to induce septicemia in streptozotocin (STZ) induced diabetic and nondiabetic mice Cited by: 8.

The results showed that Phage MB08 has therapeutic significance in treating burn wound infections in rats since a single topical application of this phage was able to rescue rats from infection caused by P. aeruginosa in comparison to multiple topical applications of antibiotics. Keywords: Pseudomonas aeruginosa, bacteriophage, Colistin. Keywords: Phage therapy, Pseudomonas aeruginosa, biofilm Background and rationale. Pseudomonas aeruginosa is the most common pathogen found in the lung of cystic fibrosis patients (CF). The quality of life of CF patients largely depends on the success or failure of antibiotic treatment.


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Insertion and replication of Pseudomonas aeruginosa mutator phage D3112 by Shehnaz Rehmat Download PDF EPUB FB2

Summary. D is a temperate bacteriophage of P. aeruginosa with heterogeneous sequences at one extremity of the virion DNA molecule.

Infection of strain PAO1 with phage D results in a to fold increase in the frequency of ami mutants resistant to fluoroacetamide.

Nine ami::D prophages have been mapped to distinct sites within the ami locus by Southern Cited by: Rehmat S, Shapiro JA. Insertion and replication of the Pseudomonas aeruginosa mutator phage D Mol Gen Genet. ; (3)– Royle PL, Matsumoto H, Holloway BW.

Genetic circularity of the Pseudomonas aeruginosa PAO chromosome. J Bacteriol. Jan; (1)– [PMC free article] Smith CA, Thomas by: KEYWORDS: Pseudomonas aeruginosa, Pf filamentous phage, UvrD helicase, Rep helicase, histone-like HU, rolling circle replication INTRODUCTION Pseudomonas aeruginosa is a highly versatile opportunistic pathogen which is the leading cause of morbidity and mortality among cystic fibrosis (CF) patients and causes significant infection in other.

The underlying mechanisms of phage-host interactions largely remained to be elucidated. In this work, Pseudomonas aeruginosa phage C11 was first characterized as a Myoviridae virus having a linear Cited by: Pseudomonas aeruginosa, an increasingly prevalent opportunistic human pathogen, is the most common gram-negative bacterium found in nosocomial (hospital-acquired) infections.P.

aeruginosa is responsible for 16% of nosocomial pneumonia cases, 12% of hospital-acquired urinary tract infections, 8% of surgical wound infections, and 10% of bloodstream infections. Pseudomonas aeruginosa phages whose DNA structure is similar to Mu1 phage DNA.

General description, localization of endonuclease-sensitive sites in DNA, and the structure of D phage homoduplexes.

Genet (in Russian). Bacteriophage D is a Mu-like temperate transposable phage of Pseudomonas aeruginosa. Genetic mapping and DNA sequence analysis have identified the left end of the phage genome as encoding the. Pseudomonas aeruginosa strain P15 produces not only pyocin R1 and phage PS10, but also a substance having a flexuous rod structure, the nature of which is so far unknown.

Characterization of the Lysogenic Repressor (c) Gene of the Pseudomonas aeruginosaTransposable Bacteriophage D Kirsty A. Salmon,* Orit Freedman,* Bruce W. Ritchings,† and Michael S.

DuBow*,1 *Department of Microbiology and Immunology, McGill University, Montreal, Quebec, Canada H3A 2B4; and †Department of Pharmacology. Pseudomonas is a genus of Gram-negative, Gammaproteobacteria, belonging to the family Pseudomonadaceae and containing validly described species.

The members of the genus demonstrate a great deal of metabolic diversity and consequently are able to colonize a wide range of niches. Their ease of culture in vitro and availability of an increasing number of Class: Gammaproteobacteria. Bacteria develop a broad range of phage resistance mechanisms, such as prevention of phage adsorption and CRISPR/Cas system, to survive phage predation.

In this study, Pseudomonas aeruginosa PA1. Krylov V.N., Bogush V.G., Ianenko A.S., and Kirsanov N.B.,Pseudomonas aeruginosa bacteriophages with DNA structure similar to the DNA structure of Mu1 phage. Evidence for similarity between D, B3, and B39 bacteriophages: Analysis of DNA splits by restriction endonucleascs, isolation of D and B3 recombinant by: 1.

Pseudomonas aeruginosa is a ubiquitous member of marine biofilm, and reduces thiosulfate to produce toxic hydrogen sulfide gas. In this study, lytic bacteriophages were isolated and applied to inhibit the growth of P.

aeruginosa in planktonic mode at different temperature, pH, and salinity. Bacteriophages showed optimal infectivity at a multiplicity of infection of 10 in saline Cited by: 4. The purpose of this study was to further characterize the Pseudomonas aeruginosa phage B3 with regards to its host range and the insertion of the phage into its host.

Attempts to infect several soil isolates, previously identified as pseudomonads, were unsuccessful. Pseudomonas. Phage Therapy, Safety Measures, Pseudomonas aeruginosa 1. Introduction Bacteria of Pseudomonas aeruginosa species have very high capability for adaptation and survival in different conditions [1] [2].

They belong to the most frequent opportunistic pathogens causing different hospital infec-File Size: 3MB. The nucleotide sequence ( bp) of the left end of the Mu-like transporable bacteriophage D cts15 from Pseudomonas aeruginosa was determined.

A b[p open reading fame (ORF) is located on the bottom strand (positions ), potentially encoding a polypeptide of residues (M r = ).Specific binding of Escherichia coli Integration Host Factor (IHF) to Cited by: @article{osti_, title = {Expression of phage-transposons of Pseudomonas aeruginosa in cells of Pseudomonas putida PpGl.

Zygotic induction, an essential condition for the emergence of defective lysogens}, author = {Gorbunova, S.A. and Akhverdyan, V.Z.

and Krylov, V.N.}, abstractNote = {The presence of a large number of clones, which have lost the ability to. Φ6 (Phi 6) is the best-studied bacteriophage of the virus family infects Pseudomonas bacteria (typically plant-pathogenic P.

syringae).It has a three-part, segmented, double-stranded RNA genome, totalling ~ kb in length. Φ6 and its relatives have a lipid membrane around their nucleocapsid, a rare trait among is a lytic phage, Family: Cystoviridae.

Since the D B replication func- tion has been genetically mapped to between and kb on the D genome [4], ORF B is most likely the D B gene. Mu B, which is required for optimal levels of phage Mu transposition in vitro and in vivo, has ATPase activity [20,21] and is a sequence-independent DNA-bind- ing protein [22].Cited by: 6.

Multidrug-resistant bacteria are the cause of an increasing number of deadly pulmonary infections. Because there is currently a paucity of novel antibiotics, phage therapy—the use of specific viruses that infect bacteria—is now more frequently being considered as a potential treatment for bacterial infections.

Using a mouse lung-infection model caused by a multidrug. Phage isolation and sequencing. We screened composting samples from the São Paulo Zoo Park (São Paulo, Brazil) [] for phages infecting Pseudomonas aeruginosa PA14, in order to access a slice of the cultivable phage diversity in this complex microbial new phages were isolated, which we named Pseudomonas phage ZC01, ZC03 and ZC Cited by: 8.phage to control P.

aeruginosa infection. Keywords: Bacteriophage, Phage therapy, Pseudomonas aeruginosa. Introduction Pseudomonas aeruginosa is a facultatively anaerobic, non-fermentative, gram-negative, rod-shaped bacterium very common in the hospital environment.

This organism is an important.2 22 23 Abstract 24 Coevolution between bacteriophages and their prey is the result of mutualistic we 25 show that pseudolysogeny is a frequent outcome of infection by virulent phages of Pseudomonas 26 aeruginosa, and that selection of resistant bacterial mutants is favored by continuous production of 27 investigated the frequency and File Size: KB.